Immunohistochemical diagnosis of immunemediated dermatoses in domestic dogs

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Mario Adán Bedolla Alva
Gerardo Salas Garrido
Fernando Constantino Casas

Abstract

A retrospective study of 28 dogs skin samples was carried out between 2001 and 2004. The fixed paraffin-embedded tissues showed microscopic lesions and suggestive diagnoses of pemphigus or lupus erythematosus (LE). The samples were re-evaluated by immunohistochemistry to identify intratissular deposits of canine IgG. Only 57.14% (16 cases) were positive. The location of IgG in the interstice space of the epidermis or in the basal membrane was helpful to differentiate between pemphigus and lupus erythematosus and among the different forms of pemphigus. From the positive cases 50% (eight cases) were consistent with pemphigus foliaceus, 31.25% (five cases) with pemphigus vulgaris, 12.5% (two cases) with pemphigus erythematosus, and 6.25% (one case) with lupus erythematosus. Immunohistochemistry is a valuable diagnostic tool for the definite diagnosis of immune mediated diseases such as pemphigus and lupus erythematosus. The aim of the present study was to demonstrate the usefulness of a never used method in Mexico for the specific diagnosis of pemphigus and lupus erythematosus.

Keywords:
immunohistochemistry pemphigus lupus erythematosus dogs skin

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