Using a quick dye for the diagnosis of microsporidiosis

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Luis Edgar Rodríguez Tovar
Heidi Giselle Rodríguez Ramírez
Yazel Valdez Nava
Alicia Magdalena Nevárez Garza
Rafael Ramírez Romero

Abstract

Cystic-like structures similar to xenomas containing ovoid or piriform organisms were observed in brain and kidney of a one-month-old rabbit. Different dyes such as H&E, PAS, toluidine blue and trichrome blue were tested. Trichrome blue yielded the best results in terms of specificity, resolution and confirmation of microsporidia spores. This dye is easily adaptable to paraffin-embedded samples by not requiring additional equipment; it is rapid, cheap and could be used as a confirmatory technique for other microsporidiosis.
Keywords:
TRICHROME BLUE RABBIT Encephalitozoon cuniculi H&E PAS

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