<em>Mycobacterium avium</em> subsp. <em>paratuberculosis</em> down-regulates mRNA expression of iron-induced macrophage Ferroportin 1

Main Article Content

Bertha Landeros-Sánchez
Jose Angel Gutierrez-Pabello
Gerardo Enrique Medina-Basulto
Tomas Benjamin Renteria-Evangelista
Efrén Díaz-Aparicio
Sawako Oshima

Abstract

Veterinaria México OA
ISSN: 2448-6760

Cite this as:

  • Landeros Sánchez B, Gutierrez Pabello JA, Medina Basulto GE, Renteria Evangelista TB, Díaz Aparicio E, Oshima S. Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1. Veterinaria México OA. 2016;3(1). doi: 10.21753/vmoa.3.1.361

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease. The mechanisms by which MAP is able to adapt to the innate host response are still unclear. We examined Ferroportin 1 (FPN1) mRNA expression levels via real-time PCR of the mouse macrophage cell line J774 that was incubated in the presence of Mycobacterium avium subsp. paratuberculosis (MAP) or MAP crude protein extract. Infection with live MAP decreased FPN1 mRNA levels in a multiplicity of infection (MOI)-dependent fashion. Macrophages infected with MOIs of 20:1 and 15:1 did not show any change in FPN1 gene expression, whereas MOIs of 10:1 and 5:1 induced a decrease of 50 and 80%, respectively. Macrophages treated with 50, 100, 150 and 200 µg/mL of MAP crude extract (ATCC19698) decreased FPN1 mRNA expression by 25%. Additionally, up-regulation of FPN1 mRNA by an iron overload treatment of 400 µM of ferric nitrilotriacetate (FeNTA) was abrogated by live MAP (MOI 20:1) by approximately 70%. Our data revealed an inhibitory effect of MAP on FPN1 mRNA and suggested a bacterial mechanism that may play a role in host iron regulation.

Figure 1. Infection by Mycobacterium avium subsp. paratuberculosis (MAP) down-regulates macrophage fpn1 gene expression. Murine macrophages, J774, were infected with MAP at different multiplicities of infection (MOIs) for 6 hours. Total RNA was extracted and gene expression was measured by real-time PCR. Results were normalized to GAPDH and expressed as units of relative expression (URE). Results are the mean ± standard deviation of 3 independent experiments with 3 replicates each. Statistical significance, P = 0.05.
Keywords:
Gene expression Johne’s disease Ferroportin 1 Iron Macrophages.

Article Details

Author Biographies

Bertha Landeros-Sánchez, Universidad Autónoma de Baja California

Profesor investigador de tiempo completo nivel C

Jose Angel Gutierrez-Pabello, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México

Profesor Investigador de Tiempo Completo

Gerardo Enrique Medina-Basulto, Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California

Profesor Investigador de Tiempo completo

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