Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle

Félix Leao Rodríguez Fierros, Gerardo Quiroz Rocha, Luis Núñez Ochoa, Adolfo Aparicio Cecilio, Eligio Gabriel Salgado Hernández

Abstract


Veterinaria México OA
ISSN: 2448-6760

Cite this as:

  • Rodríguez Fierros FL, Quiroz Rocha G, Núñez Ochoa L, Aparicio Cecilio A, Salgado Hernández EG. Practical use of clinical cytology for the diagnosis of hepatic lipidosis in dairy cattle. Veterinaria México OA. 2018;5(1). doi: 10.21753/vmoa.5.1.386.

Hepatic lipidosis is a metabolic disorder that occurs frequently in dairy cattle. Its clinical evaluation on dairy farms is complex, as it is based on physi­cal, chemical and biological tests. The present study evaluates the practical use of hepatic cytology in dairy cattle and suggests that overall health and dairy production rates of animals are not compromised with this procedure. Cell and blood samples were obtained from 25 randomly selected multipa­rous Holstein dairy cows at three different times during the transition period (at calving and at 10 and 21 days postpartum). The values of haematocrit, plasma protein, fibrinogen, and total and differential leucocyte counts were determined, together with total protein, albumin, glutamate dehydrogenase, triacylglycerides, cholesterol and ß-hydroxybutyrate. Cytological results were classified by the detected degree of fatty infiltration, and haematological and metabolic results were compared between each sampled period. The health status and the daily milk production at each sampling stage were recorded. Obtained results indicated that the animals did not show significant clinical inflammation or a decrease in dairy production, which could be deemed secondary to the cytological sampling. This technique is thus regarded as a feasible alternative for the diagnosis of hepatic lipidosis in dairy cattle.

Figure 1. Cell aspiration from cow liver in 11th right intercostal space approximately 15 to 20 cm below the spinous apophysis.

Keywords


Lipidosis, cytology, dairy cow, fatty infiltration

Full Text:

PDF HTML

References


Stöber M. Enfermedades del hígado. In: Dirksen G, Gründer HD, Stöber R, editors. Medicina interna y cirugía del bovino. 4th edition, vol. 1(1). Buenos Aires (AR): Inter-Médica, 2005; p. 589-605.

den Boer M, Voshol P, Kuipers F, et al. Hepatic Steatosis: A Mediator of the Metabolic Syndrome. Lessons From Animal Models. Arterioscler Thromb Vasc Biol. 2004;24:644-9. doi: /10.1161/01.ATV.0000116217.57583.6e

Vernon RG. Lipid metabolism during lactation: A review of adipose tissue-liver interactions and the development of fatty liver. J Dairy Res, 2005;72:460-9. doi: 10.1017/S0022029905001299

Mulligan FJ, Doherty ML. Production diseases of the transition cows. Vet J, 2008;176:3-9. doi:10.1016/j.tvjl.2007.12.018

Bobe G, Young JW, Beitz DC. Invited review: Pathology, etiology, prevention and treatment of fatty liver in dairy cows. J Dairy Sci, 2004;87:3105-24. doi: 10.3168/jds.S0022-0302(04)73446-3

Quiroz-Rocha GF, Bouda J. Patología clínica de hígado. In: Nuñez Ochoa L, Bouda J, editors. Patología clínica veterinaria. DF (MX): UNAM, 2007; p. 122-37.

Starke A, Haudum A, Weijers G, et al. Technical note: analysis of total lipids and triacylglicerol content in small liver biopsy samples in cattle. J Anim Sci, 2010;88:2741-50. doi: 10.2527/jas.2009-2599

Jorritsma R, Jorritsma H, Schukken YH, et al. Prevalence and indicators of post partum fatty infiltration of the liver in nine commercial herds in the Netherlands. Livest Prod Sci, 2001;68:53-60. doi: 10.1016/S0301-6226(00)00208-6

Reid IM. Incidence and severity of fatty liver in dairy cows. Vet Rec, 1980;107:281-4. doi: 10.1136/vr.107.12.281

Eddy RG. Metabolics disease. In: Andrews AH, Boyd H, Eddy RG, Blowey RW, editors. Bovine medicine disease and husbandry of cattle. Oxford (UK): Blackwell Science, 2004;801-3.

Kalaitzakis E, Roubies N, Panousis N, Pourliotis K, Kaldrymidou E, Karatzias H. Clinicopathologic evaluation of hepatic lipidosis in periparturient dairy cattle. J Vet Intern Med, 2007;21:835-45. doi: 10.1111/j.1939-1676.2007.tb03029.x

Quiroz-Rocha GF, Leblanc S, Duffield T, et al. Evaluation of prepartum serum cholesterol and fatty acids concentrations as predictors of pospartum retention of the placenta in dairy cows. J Am Vet Med Assoc, 2009;234:790-793. doi: 10.2460/javma.234.6.790

LeBlanc S. Monitoring metabolic health of dairy cattle in the transition period. J Reprod Dev, 2010;56:S29-35.

Rosciani AS, Merlo WA, Insfrán RM, Ruíz Díaz Benitez, Locket M, Koscinkzu P. Caracterización citológica de hepatopatías en perros y gatos. Rev Vet, 2007;18:111-116.

Rosendo O, Mcdowell LR. Liver dry matter and liver lipids in periparturient dairy cows. Act Vet Brno, 2003;72:541-546.

Herdt TH, Goeders L, Emery RS, et al. Test for estimation of bovine hepatic lipid content. J Am Vet Med Assoc, 1983;182:953-955.

Bohn AA, Callan RJ. Cytology in food animal practice. Vet Clin N Am-Food A P, 2007;23:443-479. doi: 10.1016/j.cvfa.2007.07.007

Bauchart D. Lipid absorption and transport in ruminants. J Dairy Sci, 1993;76:3864-3881. doi: 10.3168/jds.S0022-0302(93)77728-0

Bauchart D, Gruffat D, Durand D. Lipid absorption and hepatic metabolism in ruminants. P Nutr Soc, 1996;55:39-47. doi: 10.1079/PNS19960010

Engelking LR. Lipid metabolism. In: Engelking LR, editor. Physiological chemistry: textbook of veterinary. Oxford (UK): Jackson, 2004:298-410.

Reid IM, Dew AM, Williams LA. Hematology of subclinical fatty liver in dairy cows. Res Vet Sci, 1984;37:63-65.

Zerbe H, Schneider N, Leibold W, Wensing T, Kruip TAM, Schuberth HJ. Altered funtional and immunophenotypical properties of neutrophilic granulocytes in postpartum cows associated with fatty liver. Theriogenology, 2000;54:771-786. doi: 10.1016/S0093-691X(00)00389-7

Wood D and Quiroz-Rocha GF. Normal hematology of cattle. In: Weiss DJ and Wardrop J, editors. Schalm’s veterinary hematology. Iowa (US): Blackwell, 2000:829-834.

Weiss DJ and Perman V. Assessment of the hematopoietic system in ruminants. Vet Clin N Am Food A P, 1992;8:411-428. doi: 10.1016/S0749-0720(15)30732-5

West HJ. Effect on liver function of acetonaemia and the fat cow syndrome in cattle. Res Vet Sci, 1990;48:221-227.

Sevinc M, Basoglu A, Oztok I, Sandikci M, Birdane F. The clinical-chemical parameters, serum lipoprotein and fatty infiltration of the liver in ketotic cows. Turk J Vet Anim Sci, 1998;22:443-447.

Reid IM, Roberts CJ. Subclinical fatty liver in dairy cows. Irish Vet J, 1983;37:104-110.

Rodríguez-Fierros FL. Uso y estandarización de la citología hepática como herramienta clínica para el diagnóstico de lipidosis hepática en vacas lecheras (Master thesis). DF (MX): UNAM, 2013.




DOI: http://dx.doi.org/10.21753/vmoa.5.1.386

Refbacks

  • There are currently no refbacks.