Establishment and maintenance of an R6/1 transgenic mouse colony and validation of its progressive neurological phenotype to study Huntington’s disease

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Lucía Gabriela García-Lara
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, 07360, México
http://orcid.org/0000-0002-9793-0145
Adriana Morales-Martínez
Laboratorio de Investigación de Bioquímica y Biofísica Computacional, Escuela Nacional de Medicina y de Homeopatía Instituto Politécnico Nacional, México. Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269, Insurgentes Sur #3877. Col. La Fama. Del. Tlalpan, C.P. 14269 Ciudad de México."
http://orcid.org/0000-0001-9475-0491
Quetzalli Denisse Angeles-López
Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, 07360, México
http://orcid.org/0000-0002-9874-1864
Hilda Pedraza-Espitia
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269, Insurgentes Sur #3877. Col. La Fama. Del. Tlalpan, C.P. 14269 Ciudad de México.
http://orcid.org/0000-0002-8881-0643
Iván Pérez-Neri
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269, Insurgentes Sur #3877. Col. La Fama. Del. Tlalpan, C.P. 14269 Ciudad de México.
http://orcid.org/0000-0003-0190-7272
Cesar Augusto Rodríguez-Balderas
Departamento de Bioterio. Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269, México.
http://orcid.org/0000-0001-8098-4094
Francisca Pérez-Severiano
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suárez, 14269, Insurgentes Sur #3877. Col. La Fama. Del. Tlalpan, C.P. 14269 Ciudad de México.
http://orcid.org/0000-0002-2840-7991

Abstract

Veterinaria México OA
ISSN: 2448-6760

Cite this as:

  • García-Lara L, Morales-Martínez A, Angeles-López QD, Pedraza-Espitia H, Pérez-Neri I, Rodríguez-Balderas CA, Pérez-Severiano F. Establishment and maintenance of an R6/1 transgenic mouse colony and validation of its progressive neurological phenotype to study Huntington’s disease. Veterinaria México OA. 2018;5(1). doi: 10.21753/vmoa.5.1.487.

Huntington’s disease (HD) is a hereditary neurodegenerative disorder of the central nervous system that mainly affects the basal ganglia and has no cure. The mutation is located at an abnormal expansion of the CAG triplet in the Huntingtin gene. Humans show psychiatric, behavioural and motor disorders. Transgenic animal models are essential to the study of HD since the disease only affects humans. Therefore, the aim of this article was to describe the formation and maintenance of and to validate the progressive neurological phenotype of an R6/1 transgenic mouse colony. To achieve our objective, the colony founder was imported from Jackson Laboratories, and the mice were kept under controlled environmental conditions. The animals were bred at the vivarium of the Instituto Nacional de Neurología y Neurocirugía Man­uel Velasco Suárez. The R6/1 transgenic mice were successfully bred and showed genetic and phenotypic characteristics similar to the ones previously reported. Our colony is currently established and validated with the condi­tions of our vivarium and has produced more than four generations of R6/1 mice. The establishment of the R6/1 colony and its maintenance through generation is an advantage since it allows us to follow the authenticity of the transgenic mice regarding their phenotypic and motor behaviours. Fur­thermore, these animals can be compared with other transgenic mice that reproduce some of the main characteristics of the disease manifested in hu­mans, making these transgenic R6/1 mice a useful tool for the study of HD.

Figure 2. D) Wt mouse with normal fur and weight.
Keywords:
Huntington’s disease motor activity R6/1 transgenic mouse colony neurodegeneration motor and behaviour measurements.
DOI: https://doi.org/10.21753/vmoa.5.1.487

Article Details

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