<em>Caesalpinia coriaria</em> fruits and leaves extracts possess <em>in vitro</em> ovicidal activity against <em>Haemonchus contortus</em> and <em>Haemonchus placei</em>
Article Sidebar
Main Article Content
Abstract
Veterinaria México OA
ISSN: 2448-6760
Cite this as:
- Rojo-Rubio R, González-Cortazar M, Olmedo-Juárez A, Zamilpa A, Arece-García J, Mendoza-Martínez GD, Lee-Rangel HA, Vázquez-Armijo JF, Mendoza-de Gives P. Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei. Veterinaria México OA. 2019;6(4). doi: 10.22201/fmvz.24486760e.2019.4.601.
The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriariatree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC50) and 90% (EC90) effective concentrations for each extract were determined using a Probit analysis.A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei, respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methyl-gallate.In conclusion,hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei.
Article Details
References
Herrera-Manzanilla FA, Ojeda-Robertos NF, González-Garduño R, Cámara-Sarmiento R., Torres-Acosta J.F.J. Gastrointestinal nematode populations with multiple anthelmintic resistance in sheep farms from the hot humid tropics of Mexico. Vet Parasitol: Regional Studies and Reports. 2017;9: 29-33.
Kaplan RM, Vidyashankar AN. An inconvenient truth: global warming and anthelmintic resistance. Vet Parasitol. 2012;186:70-8.
von Son de Fernex E, Alonso-Díaz MA, Mendoza de Gives P, Valles de la Mora B, Zamilpa A, González-Cortazar M. Ovicidal activity of extracts from four plant species against the cattle nematode Cooperia puncata. Vet Mex. 2016; 3(2): 1-14.
Olmedo-Juárez A, Rojo-Rubio R, Zamilpa A, Mendoza-de Gives P, Arece-García J, López-Arellano ME, et al. In vitro larvicidal effect of a hydroalcoholic extract from Acacia cochliacantha leaf against ruminant parasitic nematodes. Vet Res Commun. 2017;41:227-32.
von Son-de Fernex E, Alonso-Díaz MA, Valles-de la Mora B, Mendoza-de Gives P, González-Cortazar M, Zamilpa A. Anthelmintic effect of 2H-chromen-2-one isolated from Gliricidia sepium against Cooperia punctata. Exp Parasitol. 2017;178:1-6.
Williams AR, Ropiak HM, Fryganas C, Desrues O, Mueller-Harvey I, Thamsborg SM. Assessment of the anthelmintic activity of medicinal plant extracts and purified condensed tannins against free-living and parasitic stages of Oesopha-gostomumdentatum. Parasite Vector. 2014;7:518-27
Klongsiriwet C, Quijada J, Williams AR, Mueller-Harvey I, Williamson EM, Hoste H. Synergistic inhibition of Haemonchus contortusexsheathment by flavonoid monomers and condensed tannins. Int J Parasitol Drug. 2015;5:127
Castillo-Mitre GF, Olmedo-Juárez A, Rojo-Rubio R, Cortazar-González M, Mendoza-de Gives P, Hernández-Beteta E.E, et al. Caffeoyl and coumaroyl derivatives from Acacia cochliacantha exhibit ovicidal activity against Haemonchus contortus. J Ethnopharmacol. 2017;204:125-31.
Zanin JL, de Carvalho BA, Martineli PS, dos Santos MH, Lago JH, Sartorelli P, et al. The genus Caesalpinia L. (Caesalpiniaceae): phytochemical and pharmacological characteristics. Molecules. 2012;17:7887-90.
López J, Tejada I, Vázquez C, De Dio G, Shimada A. Condensed tannins in humid tropical fodder crops and their in vitro biological activity: Part 2. J Sci Food Agric. 2004;84: 295-9.
Olmedo-Juárez A, Rojo-Rubio R, Arece-García J, Salem AZM, Kholif EA, Morales-Almaraz E. In vitro of Pithecellobium dulce and Lysiloma acapulcensis on the exogenous development of gastrointestinal strongyles in sheep. Ital J Anim Sci. 2014;13:303-7.
Porter LJ, Hirstich LN, Chan BG. The conversion of procyanidins and prodelphinidins to cyanidin and delhpinidin. Phytochem. 1986;25:223-30.
Hedqvist H, Mueller-Harvey I, Reed JD, Krueger CG, Murphy M. Characterization of tannins and in vitro protein digestibility of several Lotus corniculatus varieties. Anim Feed Sci Tech. 2000;87:41-56.
Wagner H, Bladt S. Plant drug analysis, a thin layer chromatography, atlas. 2nd ed. Berlin, Heidelberg, New York: Springer-Verlag; 1996.
Coles GC, Bauer C, Borgsteede F, Geerts S, Klei TR, Taylor MA, et al. World association for advancement in veterinary parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol. 1992;44:35-43.
Cortes-Morales J, Olmedo-Juárez A, Trejo-Tapia G, González-Cortazar M, Domínguez-Mendoza BE, Mendoza de Gives P, et al. In vitro ovicidal activity of Baccharis conferta Kunth against Haemonchus contortus. Exp Parasitol. 2019;197:20-8.
SAS Institute, SAS User ́s Guide: Statistics, Ver 9.0., SAS Institute, Cary, NC USA; 2006.
Steel RGD, Torrie JH, Dickey DA. Principles and procedures of statistics: a biometrical approach. 3rd ed. New York: McGraw-Hill; 1997.
García-Hernández C, Rojo-Rubio R, Olmedo-Juárez A, Zamilpa A, Mendoza de Gives P, Antonio-Romo IA, et al. Galloyl derivatives from Caesalpiniacoriaria exhibit in vitro ovicidal activity against cattle gastrointestinal parasitic nematodes. Exp Parasitol. 2019;200:16-23. https://doi.org/10.1016/j.exppara.2019.03.012.
Sánchez-Carranza JN, Alvarez L, Marquina-Bahena S, Salas-Vidal E, Cuevas V, Jiménez EW, et al. Phenolic compounds isolated from Caesalpinia cori-aria induce S and G2/M phase cell cycle arrest differentially and trigger cell death by interfering with microtubule dynamics in cancer cell lines. Molecules. 2017;22(4):666. https://doi.org/10.3390/molecules22040666.
Tehrani A, Javanbakht J, Jani M, Sasan F, Solat, A, Rajabian M, et al. Histopathological study of Haemonchus contortus in Herrik sheep abomasum. J Bacteriol Parasitol. 2012;3:144. doi: 10.4172/2155-9597.10001.
Taylor MA, Coop RL, Wall RL. Veterinary parasitology. 4th ed. Ames, IA: Wiley-Blackwell; 2016. p. 364-8.
Bukke AN, Hadi FN, Produtur CS. Comparative study of in vitro antibacterial activity of leaves, bark, heart wood and seed extracts of Caesalpinia sappan L. Asian Pac J Trop Dis. 2015;5:903-7.
Dong R, Yuan J, Wu S, Huang J, Xu X, Wu Z, et al. Anti-inflammation furanoditerpenoids from Caesalpinia minax Hance. Phytochemistry. 2015;117:325-31.
von Son-de Fernex E, Alonso-Díaz MA, Valles-de la Mora B, Capetillo LCM. In vitro anthelmintic activity of five tropical legumes on exsheathment and motility of Haemonchus contortus infective larvae. Exp Parasitol. 2012;131:413-8.
Akkari H, Hajaji S, B’chir F, Rekik M, Gharbi M. Correlation of polyphenolic content with radical-scavenging capacity and anthelmintic effects of Rubus ulmifoli-us (Rosaceae) against Haemonchus contortus. Vet Parasitol. 2016;221:46-53.
García-Hernández C, Arece-García J, Rojo-Rubio R, Mendoza-Martínez GD, Albarrán-Portillo B, Vázquez-Armijo JF, et al. Nutraceutic effect of free condensed tannins of Lysiloma acapulcensis (Kunth) benth on parasite infection and per-formance of Pelibuey sheep. Trop Anim Health Prod. 2017;49:55-61.
Jabbar A, Zaman MA, Iqbal Z, Yaseen M, Shamim A. Anthelmintic activity of Chenopodium album (L) and Caesalpinia crista (L) against trichostrongylid nematodes of sheep. J Ethnopharmacol. 2007;114:86-91.
Falcão L, Araújo MEM. Tannins characterization in new and historic vegetable tanned leathers fibres by spot tests. J Cult Herit. 2011;12:149-56.
Brunet S, Fourquaux I, Hoste H. Ultrastructural changes in the third-stage, infective larvae of ruminant nematodes treated with sainfoin (Onobrychis viciifolia) extract. Parasitol Int.2011;60:419-24.
Tibe O, Sutherland IA, Lesperance L, Harding DRK. The effect of purified condensed tannins of forage plants from Botswana on the free-living stage of gastrointestinal nematode parasites of livestock. Vet Parasitol. 2013;197:160-7.
Jourdes M, Pouységu L, Deffieux D, Teissedre PL, Quideau S. Hydrolyzable tannins: gallotannins and ellagitannins. In: Ramawat K, Mérillon JM. (Eds.). Natural Products. 2013; Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22144-6_65
De Jesús-Martínez X, Olmedo-Juárez A, Olivares-Pérez J, Zamilpa A, Mendoza de Gives P, López-Arellano ME, et al. In vitro anthelmintic activity of methanolic extract from Caesalpinia coriaria J. Willd fruits against Haemonchus contortuseggs and infective larvae. BioMed Res Int. 2018;2018:7375693. https://doi.org/10.1155/2018/7375693.
Pérez MA, Rengifo R, Pereira C, Hernández V. Dividivi tannins: an ecological product for water-baser drilling fluids. Environ Dev Sustain. 2017;19:1815-29. https://doi.org/10.1007/s10668-016-9829-0
Sánchez N, Mendoza GD, Martínez JA, Hernández PA, Camacho Diaz LM, Lee-Rangel HA, et al. Effect of Caesalpinia coriariafruits and soybean oil on finishing lamb performance and meat characteristics. BioMed Res Int. 2018;2018:9486258. https://doi.org/10.1155/2018/9486258
Alonso-Díaz MA, Torres-Acosta JFJ, Sandoval-Castro CA, Capetillo-Leal CM. Polyphenolic compounds of nutraceutical trees and the variability of their biological measured by two methods. Trop Sutrop Agroecosyst. 2010;12:649-56
von Son-de Fernex E, Alonso-Díaz MA, Mendoza-de-Gives P, Valles-de la Mora B., González-Cortazar M, Zamilpa A, et al. E. Elucidation of Leucaenaleucocephala anthelmintic-like phytochemicals and the ultrastructural damage generated to eggs of Cooperia spp. Vet Parasitol. 2015;214:89-95.
Zarlenga DS, Hoberg EP, Tuo W. Chapter five. The identification of Haemonchus species and diagnosis of haemonchosis. Adv Parasitol. 2016;93:145-80. https://doi.org/10.1016/bs.apar.2016.02.023
Mansfield LS, Gamble HR, Fetterer RH. Characterization of the eggshell of Haemonchus contortus – I. Structural components. Comp Biochem Physiol. 1992;103B:681–6.
License
Veterinaria México OA by Facultad de Medicina Veterinaria y Zootecnia - Universidad Nacional Autónoma de México is licensed under a Creative Commons Attribution 4.0 International Licence.
Based on a work at http://www.revistas.unam.mx
- All articles in Veterinaria México OA re published under the Creative Commons Attribution 4.0 Unported (CC-BY 4.0). With this license, authors retain copyright but allow any user to share, copy, distribute, transmit, adapt and make commercial use of the work, without needing to provide additional permission as long as appropriate attribution is made to the original author or source.
- By using this license, all Veterinaria México OAarticles meet or exceed all funder and institutional requirements for being considered Open Access.
- Authors cannot use copyrighted material within their article unless that material has also been made available under a similarly liberal license.